Coding
tANK6
Part:BBa_K3955105:Design
Designed by: Camilla Lane Group: iGEM21_Lund (2021-09-30)
tANK6 peptide fused with slp_mod signal peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order for tANK6 to distinguishable from SLPmod it needed to be longer than it was. We therefore repeated the sequence 3 times and introduces cleavege sites for pancreatic elastase in between the repeats. Pancreatic elastase was chosen as a peptidase since it naturally occurs in the colon, which would be the target organ of Team lund 2021s potential future implementation. Codons were chosen manually to reduce the sequence repeats in the nucleotide sequence but still mainly use the most common codons for L. reuteri, our intended chassi.
Source
tANK6 is a peptide derived from csgA, identified by XX.
Lb. reuteri Mub .... and the sequence for it was taken from the parts registry.